Question

Looks like we are all out of Basic Germination Media!!! How will be able to perform hanging drop experiments

now?

Luckily we know what Basic Germination Media consists of; we know it is made up of 1mM KCl, 0.1mM CaCl2, 1.6mM H3BO3, and 10% Glucose. We also have stock solution of each component consisting of 150mM KCl, 100mM CaCl2, 20mM H3BO3, and 20% Glucose solution.

How much of each solution will you need to make 10mL of Basic Germination Media?

Make sure to include the units

KCl ?

CaCl2 ?

H3BO3 ?

Glucose ?

Water ?

Science