From a kinetics experiment kcat was determined to be 3 5 x 103sec1

For the

Question

From a kinetics experiment, kcat was determined to be 3.5 x 103sec-1. For the

kinetic assay, 0.15mL of a 0.5mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 45,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (µM∙min-1) for the enzyme and catalytic efficiency ( in M-1sec-1) for the enzyme. The Kmfor the enzyme was determined to be 6.5 x 10-2M.

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