1 Name the color substrate used explain how the blue color is formed in the presence of the antibody bound to the

Question

1.Name the color substrate used explain how the blue color is formed in the presence of the antibody bound to the

well

2.What is the enzyme that is linked to the antibody in an ELISA test

3.What is the blocking agent used in the ELISA experiment and explain what would happen if you skip this step

4.What is the substance present in the media that induces expression of the GFP protein from the pGLO plasmid

5.Name one media that will only allow Gram positive bacteria, but not Gram negative bacteria to grow (1pt) and describe how this media select for gram positive bacteria

6.What is the dye and sugar present in MSA (Mannitol Sa Agar) and why would it turn yellow after incubation with Staphylococcus aureus

7.Name one type of agar plate that you can use to differentiate between Staphylococcus aureus and Staphylococcus epidermidis and how that could be done

8.Outline the gram stain procedure and indicate what color is positive and what is negative for the outcome

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